REPOPULATION OF THE POSTMITOTIC NUCLEOLUS BY PREFORMED RNA
نویسندگان
چکیده
منابع مشابه
Repopulation of the Postmitotic Nucleolus by Preformed Rna
This study is concerned with the fate of the nucleolar contents, particularly nucleolar RNA, during mitosis Mitotic cells harvested from monolayer cultures of Chinese hamster embryonal cells, KB6 (human) cells, or L929 (mouse) cells were allowed to proceed into interphase in the presence or absence (control) of 0.04-0 08 microg/ml of actinomycin D, a concentration which preferentially inhibits ...
متن کاملRepopulation of Postmitotic Nucleoli by Preformed Rna
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis. When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as u...
متن کاملREPOPULATION OF POSTMITOTIC NUCLEOLI BY PREFORMED RNA II . Ultrastructure
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis . When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as ...
متن کاملReformation of Nucleolus-like Bodies in the Absence of Postmitotic Rna Synthesis
The dependence of nucleolar reformation on RNA synthesis that resumes in late anaphase or early telophase has been investigated in synchronously dividing Amoeba proteus. RNA synthesis was completely inhibited throughout all stages of mitosis and the early hours of interphase with high concentrations of actinomycin D. In such cells, nucleolus-like bodies that bind azure B and pyronin were appare...
متن کاملThe Dynamics of Postmitotic Reassembly of the Nucleolus
Mammalian cell nucleoli disassemble at the onset of M-phase and reassemble during telophase. Recent studies showed that partially processed preribosomal RNA (pre-rRNA) is preserved in association with processing components in the perichromosomal regions (PRs) and in particles called nucleolus-derived foci (NDF) during mitosis. Here, the dynamics of nucleolar reassembly were examined for the fir...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.53.3.611